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anti mef2d mouse  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti mef2d mouse
    Anti Mef2d Mouse, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 55 article reviews
    anti mef2d mouse - by Bioz Stars, 2026-10
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    Related Articles

    Isolation:

    Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling
    Article Snippet: Chromatin immunoprecipitation Native protein-DNA complexes were cross-linked by treatment with 1% formaldehyde for 15 min. A SimpleChIP Plus Enzymatic Chromatin IP kit (Cell Signaling Technology) was used according to the manufacturer’s protocol. .. Briefly, equal aliquots of isolated chromatin were subjected to immunoprecipitation with anti-Mef2D antibody (Santa Cruz Biotechnology) or mouse immunoglobulin G control (Active Motif). ..

    Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling.
    Article Snippet: Native protein–DNA complexes were cross-linked by treatment with 1% formaldehyde for 15 min. A SimpleChIP Plus Enzymatic Chromatin IP kit (Cell Signaling Technology) was used according to the manufacturer’s protocol. .. Briefly, equal aliquots of isolated chromatin were subjected to immunoprecipitation with anti-Mef2D antibody (Santa Cruz Biotechnology) or mouse immunoglobulin G control (Active Motif). ..

    Immunoprecipitation:

    Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling
    Article Snippet: Chromatin immunoprecipitation Native protein-DNA complexes were cross-linked by treatment with 1% formaldehyde for 15 min. A SimpleChIP Plus Enzymatic Chromatin IP kit (Cell Signaling Technology) was used according to the manufacturer’s protocol. .. Briefly, equal aliquots of isolated chromatin were subjected to immunoprecipitation with anti-Mef2D antibody (Santa Cruz Biotechnology) or mouse immunoglobulin G control (Active Motif). ..

    Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling.
    Article Snippet: Native protein–DNA complexes were cross-linked by treatment with 1% formaldehyde for 15 min. A SimpleChIP Plus Enzymatic Chromatin IP kit (Cell Signaling Technology) was used according to the manufacturer’s protocol. .. Briefly, equal aliquots of isolated chromatin were subjected to immunoprecipitation with anti-Mef2D antibody (Santa Cruz Biotechnology) or mouse immunoglobulin G control (Active Motif). ..

    Control:

    Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling
    Article Snippet: Chromatin immunoprecipitation Native protein-DNA complexes were cross-linked by treatment with 1% formaldehyde for 15 min. A SimpleChIP Plus Enzymatic Chromatin IP kit (Cell Signaling Technology) was used according to the manufacturer’s protocol. .. Briefly, equal aliquots of isolated chromatin were subjected to immunoprecipitation with anti-Mef2D antibody (Santa Cruz Biotechnology) or mouse immunoglobulin G control (Active Motif). ..

    Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling.
    Article Snippet: Native protein–DNA complexes were cross-linked by treatment with 1% formaldehyde for 15 min. A SimpleChIP Plus Enzymatic Chromatin IP kit (Cell Signaling Technology) was used according to the manufacturer’s protocol. .. Briefly, equal aliquots of isolated chromatin were subjected to immunoprecipitation with anti-Mef2D antibody (Santa Cruz Biotechnology) or mouse immunoglobulin G control (Active Motif). ..

    Incubation:

    Article Title: Transcription factor MEF2D regulates aberrant expression of ACSL3 and enhances sorafenib resistance by inhibiting ferroptosis in HCC
    Article Snippet: .. Cell lysates were pre-clarified with protein A/G beads and then incubated overnight at 4°C with protein A/G beads coated with anti-MEF2D antibody (2 μg, Santa cruz). ..

    other:

    Article Title: Loss of MEF2D expression inhibits differentiation and contributes to oncogenesis in rhabdomyosarcoma cells
    Article Snippet: Blot was probed with anti-MEF2D antibody (P-17, Santa Cruz Biotechnologies).

    Article Title: Histone demethylase LSD1 is required to induce skeletal muscle differentiation by regulating myogenic factors.
    Article Snippet: Article history: Received 30 August 2010 Available online 15 September 2010



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    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and <t>MEF2D</t> (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .
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    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and <t>MEF2D</t> (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .
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    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and <t>MEF2D</t> (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .
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    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and <t>MEF2D</t> (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .
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    Figure 3 | Adeno-associated virus was injected into the skeletal muscle of C57BL/6 mice in situ (n = 6). (a) Mouse life curve. (b) Body weight of mice in each group. (c) General pictures of mice in each group. (d) The general map and fluorescence intensity of each tissue of mice injected with the adeno-associated virus group were captured by a small animal imager. (e) mCherry protein expression in mouse tissues (skeletal muscle, heart, liver, epididymis white adipose tissue, subcutaneous white adipose tissue and brown adipose tissue). (f–i) Expression of <t>MEF2D</t> and GLUT4 in skeletal muscle tissues of mice in each group. (j, k) Glucose tolerance and area under the curve (AUC) of mice in each group. (l, m) Insulin sensitivity and area under the curve (AUC) of mice in each group. (t-test, *, compared with HFD-NC group, P < 0.05; #, compared with HFD- mimics group, P < 0.05, the difference was statistically significant).
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    Image Search Results


    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and MEF2D (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .

    Journal: iScience

    Article Title: MyoD is essential in rhabdomyosarcoma by promoting survival through differentiation and CYLD

    doi: 10.1016/j.isci.2025.113149

    Figure Lengend Snippet: RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and MEF2D (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .

    Article Snippet: Antibodies used in this study included, IκBα (Cell Signaling Technology Cat# 9242, RRID: AB_331623 ), α-tubulin (Thermo Fisher Scientific Cat# A11126, RRID: AB_2534135 ), p65/RelA (Santa Cruz Biotechnology Cat# sc-109, RRID: AB_632039 ), MyoD (Santa Cruz Biotechnology Cat# sc-377460, RRID: AB_2813894 ), Myf5 (Santa Cruz Biotechnology Cat# sc-518039), myogenin (Santa Cruz Biotechnology Cat# sc-52903, RRID: AB_784707 ), MRF4 (Santa Cruz Biotechnology sc-514379), MEF2C (Abcam Cat# ab211493, RRID: AB_2864417 ), MEF2D (Santa Cruz Biotechnology Cat# sc-271153, RRID: AB_10614669 ), MYHC (Sigma-Aldrich Cat# M8421, RRID: AB_477248 ), TnnT (Sigma-Aldrich Cat# SAB4200717, RRID: AB_2892079 ), CYLD (Cell Signaling Technology Cat# 4495, RRID: AB_10557111 ), p -RIPK1 (Proteintech Cat# 66854-1-Ig, RRID: AB_2882194 ), RIPK1 (Thermo Fisher Scientific Cat# PA5-20811, RRID: AB_11154790 ), DNMT1 (Novus Cat# NB100-56519, RRID: AB_838131 ), DNMT3A (Santa Cruz Biotechnology Cat# sc-365769, RRID: AB_10844010 ), DNMT3B (Cell Signaling Technology Cat# 67259, RRID: AB_2799723 ), and HRP conjugated secondary antibodies anti-mouse IgG (Promega Cat# W4021, RRID: AB_430834 ) and anti-rabbit IgG (Promega Cat# W4011, RRID: AB_430833 ).

    Techniques: Immunohistochemistry, Staining, Expressing, Control

    Figure 3 | Adeno-associated virus was injected into the skeletal muscle of C57BL/6 mice in situ (n = 6). (a) Mouse life curve. (b) Body weight of mice in each group. (c) General pictures of mice in each group. (d) The general map and fluorescence intensity of each tissue of mice injected with the adeno-associated virus group were captured by a small animal imager. (e) mCherry protein expression in mouse tissues (skeletal muscle, heart, liver, epididymis white adipose tissue, subcutaneous white adipose tissue and brown adipose tissue). (f–i) Expression of MEF2D and GLUT4 in skeletal muscle tissues of mice in each group. (j, k) Glucose tolerance and area under the curve (AUC) of mice in each group. (l, m) Insulin sensitivity and area under the curve (AUC) of mice in each group. (t-test, *, compared with HFD-NC group, P < 0.05; #, compared with HFD- mimics group, P < 0.05, the difference was statistically significant).

    Journal: Journal of diabetes investigation

    Article Title: Adipocyte exosome miR-4472 inhibits glucose uptake in skeletal muscle through downregulation of MEF2D.

    doi: 10.1111/jdi.70054

    Figure Lengend Snippet: Figure 3 | Adeno-associated virus was injected into the skeletal muscle of C57BL/6 mice in situ (n = 6). (a) Mouse life curve. (b) Body weight of mice in each group. (c) General pictures of mice in each group. (d) The general map and fluorescence intensity of each tissue of mice injected with the adeno-associated virus group were captured by a small animal imager. (e) mCherry protein expression in mouse tissues (skeletal muscle, heart, liver, epididymis white adipose tissue, subcutaneous white adipose tissue and brown adipose tissue). (f–i) Expression of MEF2D and GLUT4 in skeletal muscle tissues of mice in each group. (j, k) Glucose tolerance and area under the curve (AUC) of mice in each group. (l, m) Insulin sensitivity and area under the curve (AUC) of mice in each group. (t-test, *, compared with HFD-NC group, P < 0.05; #, compared with HFD- mimics group, P < 0.05, the difference was statistically significant).

    Article Snippet: ZSGB-BIO (China) provided the b-actin (ab234430) and GAPDH (TA-08) antibodies, while Proteintech (China) provided the MEF2D (PA5-30950) and GLUT4 (66846-1-Ig) antibodies.

    Techniques: Virus, Injection, In Situ, Fluorescence, Expressing